Blood And Tissues

TITLE: BLOOD & TISSUES- Staining Of Blood Films & Identification Of Leukocytes. OBJECTIVES: i.To come along the preparation of kind films from human blood sample. ii.To learn the techniques of dishonor the blood films with Giemsa filthiness and Wright territory. iii.To identify and make drawing of leukocytes that donation in human blood sample under microscope. insertion: Leukocytes ar divided into granulocytes and agranulocytes. Granulocytes be also known as polymorphonuclear leukocytes, or polys for short. Three types of granulocyte are neutrophils, bromeosinophils, basophils. Meanwhile, there are ii types of agranulocyctes which are lymphocytes and monocytes. If we observe the smear as it is after fixing, we choke off on chatter really little because all cells are very transparent. The erythrocytes are slightly visible, but the leukocytes are alike pale, just about invisible and we will not see anything inside them. To be able to observe and recogni ze the antithetical kinds of leukocyte, we must dirt them. For this purpose, normally Giemsa land and Wright stain are used. Giemsa stain is a mixture of dyes, based on methylene blue (basic dye) and eosin ( acidic dye). Giemsa stain is a classical blood film stain for peripheral blood smears and bone sum specimens.

The principle of Giemsa stain is the basic dye dye the acidic sectionalisation of the burden of the cell and the acidic dye stained the basic part of the cytoplasm of the cell. Therefore, the Giemsa stain allows clear observations to classify types of leukocytes. By patch of the cells we can o bserve the different types of whiteness bloo! d cells (leukocyctes) which are, neutrophils, eosinophils, basophils, lymphocytes and monocytes under the light microscope. MATERIALS: 1)Alcohol swabs 2)Blood Lancets 3)Deionized water 4)Distilled water 5)Giemsa stain 6)Glass Slides 7)Jar of disinfection for disposal of slides 8)Methanol 9)Microscopes 10)Oil Immersion 11)Pasteur pipettes 12)Sorensens...If you want to get a just essay, fix it on our website: OrderCustomPaper.com

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